RNA 2',3'-cyclic phosphate and 5'-OH ligase is a protein that in humans is encoded by the RTCB gene.[5] It is found in the stress granule of cells.[6]

RTCB
Identifiers
AliasesRTCB, C22orf28, DJ149A16.6, FAAP, HSPC117, RNA 2',3'-cyclic phosphate and 5'-OH ligase
External IDsOMIM: 613901; MGI: 106379; HomoloGene: 36344; GeneCards: RTCB; OMA:RTCB - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_014306

NM_145422

RefSeq (protein)

NP_055121

NP_663397

Location (UCSC)Chr 22: 32.39 – 32.41 MbChr 10: 85.77 – 85.79 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Structure

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As of June 2019, no crystal structure of the human RTCB is known, but homology models built from other RtcB-family ligases are available (Swiss-model: Q9Y3I0). The structure of Pyrococcus horikoshii RtcB, which uses GTP instead of ATP, shows two manganese (Mn2+) cofactors, and a mechanism involving a covalently linked GTP-histidine-RtcB intermediate. The residue involved, H404, is conserved in human RTCB as H428.[7]

Function

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Protein family

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tRNA splicing ligase RtcB
Identifiers
SymbolRtcB
PfamPF01139
InterProIPR001233
PROSITEPS01288
CATH1uc2
SCOP21uc2 / SCOPe / SUPFAM
Available protein structures:
Pfam  structures / ECOD  
PDBRCSB PDB; PDBe; PDBj
PDBsumstructure summary

RTCB belongs to the RtcB family of ATP-dependent RNA ligases, named after the eponymous protein in E. coli. The bacterial RtcB acts as a tRNA ligase, rejoining broken stem-loops in case of damage.[8] It is also able to catalyse RNA splicing.[9]

The eukaryotic homologs of RtcB, including the human RTCB protein, participates in the tRNA-splicing ligase complex.[10]

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000100220Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000001783Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ "Entrez Gene: RNA 2',3'-cyclic phosphate and 5'-OH ligase". Retrieved 2019-06-06.
  6. ^ Jain S, Wheeler JR, Walters RW, Agrawal A, Barsic A, Parker R (January 2016). "ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure". Cell. 164 (3): 487–98. doi:10.1016/j.cell.2015.12.038. PMC 4733397. PMID 26777405.
  7. ^ Desai KK, Bingman CA, Phillips GN, Raines RT (April 2013). "Structures of the noncanonical RNA ligase RtcB reveal the mechanism of histidine guanylylation". Biochemistry. 52 (15): 2518–25. doi:10.1021/bi4002375. PMC 3670941. PMID 23560983.
  8. ^ Tanaka N, Shuman S (March 2011). "RtcB is the RNA ligase component of an Escherichia coli RNA repair operon". The Journal of Biological Chemistry. 286 (10): 7727–31. doi:10.1074/jbc.C111.219022. PMC 3048659. PMID 21224389.
  9. ^ Tanaka N, Meineke B, Shuman S (September 2011). "RtcB, a novel RNA ligase, can catalyze tRNA splicing and HAC1 mRNA splicing in vivo". The Journal of Biological Chemistry. 286 (35): 30253–7. doi:10.1074/jbc.C111.274597. PMC 3162383. PMID 21757685.
  10. ^ Popow J, Englert M, Weitzer S, Schleiffer A, Mierzwa B, Mechtler K, et al. (February 2011). "HSPC117 is the essential subunit of a human tRNA splicing ligase complex". Science. 331 (6018): 760–4. Bibcode:2011Sci...331..760P. doi:10.1126/science.1197847. hdl:11858/00-001M-0000-0011-5682-B. PMID 21311021. S2CID 1881494.

Further reading

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